Oxytherm+多通道线粒体呼吸测定系统

先进的Clark氧电极系统——研究线粒体呼吸的科研利器

实时监测反应体系中溶解氧的浓度及变化，可多通道（最大8通道）同时检测多个样品

• 研究线粒体的I态、II态、III态、IV态呼吸，计算呼吸控制率及P/O比等；

• 研究肌肉、肝脏、大脑器官组织等细胞、细菌、酵母、藻类等微生物的呼吸（耗氧）速率；

• 研究呼吸途径（抗氰呼吸、细胞色素氧化酶途径、糖酵解途径等）；

• 研究有氧参与的酶促反应(生物反应）速率，有氧参与的化学反应；

• 研究某些化学合成物质的放氧速率。

系统特点

• 24位高分辨率10^-3pmol·ml^-1

• 电子精确控温(精度±0.5℃）

• 多通道实时显示数据（1～8通道可选）

• 样品用量少0.2～2.5ml

• 能与H⁺电极同时测定

氧电极用于测定酶活性Sevier C S，et al.Cell,2007,129(2):333-44

线粒体状态呼吸和呼吸控制率

Papathanasiou S, Rickelt S, Soriano M E, et al. Tumor necrosis factor-α confers cardioprotection through ectopic expression of keratins K8 and K18[J]. Nature Medicine,2015,21(9):1076.

氧电极用于抗体免疫研究llani T, et al. Science, 2013, 341(6141):74 

氧电极用于测定细胞呼吸Zhang H, et al. Cancer Cell, 2007, 11(5):407

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