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[科研前线|PEA]非生物胁迫对快速Chl荧光OJIP曲线参数变化影响

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荧光诱导动力学曲线中蕴藏着丰富的信息。近年来快速叶绿素荧光诱导动力学的应用，使PSII 供体侧和受体侧电子传递的研究更加深入。Strasser 和Strasser (1995)在生物膜能量流动基础上建立了针对快速叶绿素荧光诱导曲线的数据分析和处理方法：JIP-测定(JIP-test)，为深入研究光合作用原初反应提供了有力而便捷的工具。
本文关于非生物胁迫对快速Chl荧光参数变化影响的内容选自Adela M. Sánchez-Moreiras&Manuel J. Reigosa编著，Springer International Publishing AG, part of Springer Nature 2018出版的《Advances in Plant Ecophysiology Techniques》，本书中第5章详尽介绍了叶绿素荧光技术在植物生理生态研究中的最新应用及进展，关于OJIP曲线、JIP-Test方法的介绍是本章重点介绍内容。


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下表将非生物胁迫对快速Chl荧光(OJIP)参数的影响进行了简要介绍，获取详细资料可点击文章末尾“阅读原文”下载英文原版电子书（提取码：myib）。
更多不同胁迫类型相关文献目录可点击公众号底部“技术支持”→“文献目录”→“荧光仪”进行查看。
非生物胁迫对快速Chl荧光参数变化的影响


胁迫类型

荧光参数变化

参考文献

光抑制

Fv/Fm、qP下降。Fv/Fm是最典型的特征性参数。

·Srivastava et al. (1995)

·Misra et al. (2001a, b)

热胁迫/高温胁迫

Fo显著下降，电子传递效率ET下降。

·Sharkey & Schrader (2006)

·Mathur et al. (2014)

QA/RC和QB-至QA-下降

·Kalaji et al. (2016)

Fv/Fm下降，Fm下降，Fo增高

·Misra et al. (2001b)

·Chen et al. (2009)

·Mathur et al. (2011a, b)

·Brestic et al. (2013)

K峰

·Srivastava & Strasser (1995)

·Strasser et al. (2000)

·Laza’r (2006)

性能指数PI下降

·Misra et al. (2001b)

·Stefanov et al. (2011)

·Brestic et al. (2012)

·Brestic and Zivcak (2013)

冷害/低温胁迫

短时期内保持PSⅡ光化学活性

·Misra et al. (2001b)

干旱/水胁迫

性能指数PI下降

·Oukarroum et al. (2007),

·Zivcak et al. (2008)

·Boureima et al. (2012)

·Guha et al. (2013)

·Jedmowski et al. (2013)

·Zivcak et al. (2014a)

由于PSⅡ活性反应中心(RC)失活和/或天线色素尺寸增加，导致ABS/RC升高。

·Van Heerden et al. (2007)

·Gomes et al. (2012)

I～P阶段振幅降低

·Oukarroum et al. (2009)

·Ceppi et al. (2012)

盐胁迫

Fv/Fm降低，热耗散DI增加，光下PSII效率降低，光下电子传递链(ET)效率降低，光下PSII开放反应中心效率降低，供体侧损伤大于受体侧

·Misra et al. (2001a,b)

·Yang et al. (2008)

·He et al. (2009)

·Zhang and Sharkey (2009)

·Mehta et al. (2010)

·Singh-Tomar et al. (2012)

营养缺乏

缺氮：光化学效率和活性反应中心RC降低。

·Redillas et al. (2011)

·Li et al. (2012)

·Dudeja & Chaudhary (2005)

氮补充：增加性能指数PI

·Van Heerden et al. (2004)

·Li et al. (2012)

·Zivcak et al. (2014b)

缺磷：光化学效率Fv/Fm降低

·Kruger et al. (1997)

·Tsimilli Michael & Strasser (2008)

·Schweiger et al. (1996)

缺钙：光化学效率Fv/Fm降低

·Misra et al. (2001a, b)

·Lauriano et al. (2006)

·Liu et al. (2009)

缺镁

·Smethurst et al. (2005)

缺铁

·Molassiotis et al. (2006)


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